Plasmid DNA Preparation Services

Gxene Biotech has been working on molecular biology research for many years. Plasmid vector is a powerful tool for genetic engineering. The quality of DNA obtained is critical factor for genetic experimentation. We can provide comprehensive plasmid DNA preparation services from microgram to gram-scale to meet the needs of customers in research institutes and biotechnology industries .

With our upgraded DNA preparation platform, Gxene Biotech offers several different research-grade and preclinical grade plasmid production. We have rich experience in plasmid extraction and guarantee that the highest quality product will be delivered to our customers. A variety of our packages are designed to support diverse downstream applications such as transfection, antibody preparation, vaccine, and gene-therapy research, etc.

 
Service Type:
 
Gxene Biotech offers three different grades of plasmid production:
 

Options	Application
Research Grade	* Molecular cloning * Mutagenesis * Southern blotting * Library construction
Transfection Grade	* Transfection and co-transfection * Virus packaging * Protein manufacturing * Antibody production * Gene, vaccine, and gene therapy studies * Animal studies
Preclinical Grade	* Virus production * DNA vaccines * Preclinical animal studies

 
Service Process:
 

 
Quality Control:
 

QC Items	Specification
Appearance	Clear, no visible particles
A260/280	1.8≤OD260/OD280≤2.0
Plasmid concentration	provide dry powder
Residual RNA	Non-detectable by gel electrophoresis
Genomic DNA	Non-detectable by gel electrophoresis
Endotoxin Assay	Transfection grade <0.1 EU/μg; Preclinical grade<0.01 EU/μg
Bioburden Testing(optional add-on)	No growth on agar plate after 48 hours
Restriction analysis(optional add-on)	Conforming to reference pattern
Homogeneity(optional add-on)	Preclinical grade:≥ 90% supercoiled

 
Service Highlights:
 
--Rich experience in plasmid extraction
--Different type of quality grades, from research to preclinical
--Quality-assured: Strict quality management, complete manufacturing documentation
--Microgram-to-gram-scale quantity, to meet various customers' needs
--One-stop downstream services, including gene synthesis, custom cloning, or mutagenesis